Antibiotic dialogues: induction of silent biosynthetic gene clusters by exogenous small molecules
نویسندگان
چکیده
منابع مشابه
Gram-positive antibiotic biosynthetic clusters: a review
The worldwide emergence of multidrug-resistant pathogens is a serious medical concern nowadays. The need to discover new bioactive molecules active against these bacteria is crucial and is one of the main fields of research for modern microbiologists. Most natural antibiotics used in medicine are biosynthesised by Gram-positive bacteria. Recent advances in genomics and genome sequencing have sh...
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1. Experimental methods 2. Strains and plasmids used in this study (Table S1) 3. Deduced functions and sequence comparison of the tar genes (Table S2) 4. Schematic diagram of the tar gene cluster direct cloning (Figure S1) 5. Restriction mapping of pCAP01-tar and its derivatives (Figure S2). 6. Results of heterologous expression of the tar gene cluster in S. coelicolor M1146 (Figure S3) 7. Resu...
متن کاملDirect cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.
Recent developments in next-generation sequencing technologies have brought recognition of microbial genomes as a rich resource for novel natural product discovery. However, owing to the scarcity of efficient procedures to connect genes to molecules, only a small fraction of secondary metabolomes have been investigated to date. Transformation-associated recombination (TAR) cloning takes advanta...
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ژورنال
عنوان ژورنال: FEMS Microbiology Reviews
سال: 2016
ISSN: 1574-6976
DOI: 10.1093/femsre/fuw035